Detailed view for TcCLB.510949.10

Basic information

TDR Targets ID: 37392
Trypanosoma cruzi, kinatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5616 | Length (AA): 1285 | MW (Da): 143189 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00782   Dual specificity phosphatase, catalytic domain
PF13855   Leucine rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004725   protein tyrosine phosphatase activity  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0016791   phosphoric monoester hydrolase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 33 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
104 404 1vzo (A) 46 321 25.00 0.00000000037 1 0.43 -0.4
107 416 1fot (A) 87 357 28.00 0.000000000038 1 0.37 0.13
140 459 1y8g (A) 53 363 17.00 0 1 0.46 -0.35
234 404 1tki (A) 115 282 29.00 0.0000025 1 0.52 -1.13
447 714 1mq4 (A) 131 384 17.00 0 0.75 0.47 -0.98
448 714 1z57 (A) 160 478 14.00 0 0.99 0.25 -0.89
952 1174 1xku (A) 43 247 30.00 0.000021 0.78 0.44 -0.25
963 1063 1dce (A) 461 565 34.00 0.00000000054 1 0.6 -1.63
1124 1268 1m3g (A) 170 314 31.00 0 1 0.56 -0.6
1128 1267 1zzw (A) 323 464 34.00 0 1 0.71 -1.92
104 416 4gv1 (A) 147 424 25.00 0.0000019 1 0.37138 0
166 447 4rgj (A) 95 392 26.00 0.00024 1 0.396255 0.11
183 426 5hes (A) 49 280 25.00 0.000046 1 0.364683 -0.07
196 335 3mdy (A) 255 411 36.00 0.0000000065 0.89 0.299749 0.56
203 402 2bva (A) 367 574 27.00 0.000012 1 0.364442 -0.11
216 457 5li9 (A) 330 572 28.00 0.00000032 0.97 0.218127 0.95
234 404 1tki (A) 115 282 29.00 0.0000032 1 0.507874 -0.97
236 436 4tnd (A) 285 486 26.00 0.000017 0.95 0.38622 0.03
479 715 5lqf (A) 30 289 28.00 0.0000013 1 0.301236 0.51
480 710 3biz (A) 323 566 19.00 0 0.74 0.365467 -0.34
497 597 3lm5 (A) 77 181 36.00 0.000056 0.97 0.396399 0.07
676 905 3mwx (A) 16 226 25.00 0.74 0.77 0.156788 1.67
891 1160 5a5c (A) 24 276 34.00 0 1 0.395917 0.52
941 1044 4nkh (A) 278 370 43.00 0.000077 0.75 0.411734 0.42
964 1049 1o6v (A) 272 354 33.00 0.02 1 0.529726 -0.85
965 1051 1h6u (A) 98 181 24.00 0.23 0.96 0.429404 -0.78
1101 1260 3cm3 (A) 1007 1166 22.00 0 1 0.369314 -0.48
1127 1265 4jmk (A) 161 300 32.00 0 1 0.624771 -1.62
1127 1284 2oud (A) 322 483 34.00 0 1 0.570757 -0.91
1127 1265 4yr8 (G) 159 298 40.00 0 1 0.705971 -1.4
1128 1260 3s4e (A) 67 199 30.00 0 1 0.573302 -1.28
1129 1260 5m43 (A) 7 165 32.00 0 1 0.330524 -0.26
1206 1246 2i6j (A) 91 131 34.00 0.64 0.38 0.383707 0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_138464)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0278445   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0269918   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0270688   leucine-rich repeat-containing protein
Entamoeba histolytica EHI_099820   leucine rich repeat and phosphatase domain containing protein
Leishmania braziliensis LbrM.20.1690   dual specificity protein phosphatase, putative
Leishmania donovani LdBPK_341950.1   kinatase, putative
Leishmania infantum LinJ.34.1950   dual specificity protein phosphatase, putative
Leishmania major LmjF.34.2190   dual specificity protein phosphatase, putative
Leishmania mexicana LmxM.33.2190   dual specificity protein phosphatase, putative
Trypanosoma brucei gambiense Tbg972.4.2420   dual specificity protein phosphatase, putative
Trypanosoma brucei Tb927.4.2460   kinatase, putative
Trypanosoma brucei Tb11.v5.0751   dual specificity protein phosphatase, putative
Trypanosoma congolense TcIL3000_4_2340   kinatase, putative
Trypanosoma cruzi TcCLB.510949.10   kinatase, putative
Trypanosoma cruzi TcCLB.506559.190   kinatase, putative

Essentiality

TcCLB.510949.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 27.0% 152 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 21.7% 346 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 25.3% 245 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 21.7% 258 aa Compounds References
Patiria pectinifera Cdc2 300 aa 22.3% 256 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 24.6% 256 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0062 0.6935 0
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.3649
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0018 0.5 0.5
0.0064 0.3377 0
0.0059 1 1
0.0063 1 0.5
0.0022 0.5 0.5
0.0063 0.7244 0.2543
0.0081 1 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0092 1 0.5
0.0059 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0059 1 1
0.0066 0.3101 0
0.0091 1 0.5
0.0026 0.5 0.5
0.0088 0.4477 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0039 0.9485 0.5
0.0069 0.3067 1
0.0032 0.5 0.5
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0093 0.8828 0
0.0007 0.5 0.5
0.0033 1 0.5
0.0061 0.6883 0.5304
0.0003 0.5 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0056 1 0.5
0.0023 0.5 0.5
0.0029 0.5 0.5
0.0037 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

17 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.510949.10 (Trypanosoma cruzi), kinatase, putative
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